mmp-12 antibody (sr03-23)  (Bio-Techne corporation)

Journal: iScience

Article Title: Human monocyte-derived macrophages shift subcellular metalloprotease activity depending on their activation state

doi: 10.1016/j.isci.2024.111171

Figure Lengend Snippet:

Article Snippet: After protein transfer, membranes were blocked for 5 min in EveryBlot Blocking buffer (Cat # 12010020, Biorad) and incubated with anti-MMP-9 (Cat # AB911, R&D systems), anti-MMP-12 (Cat # AF917, R&D systems), anti-ADAM-9 (Cat # DY939, Detection antibody of Human DuoSet ELISA, R&D systems), anti-SP1 (Cat # 5931, Cell Signaling), anti-β-actin (Cat # 20536-1, Proteintech), anti-H3 (Cat # 9715, Cell Signaling), anti-CD44 (Cat # 3570, Cell Signaling), anti-HSP90 (Cat # MAB3286, R&D systems), anti-TIMP-1 (Cat # 8946S, Cell Signaling) or anti-A2M (Cat # AF1938, R&D Systems) antibodies diluted in 10% EveryBlot Blocking buffer in TBS-T (1× TBS containing 0.1% Tween 20) overnight at 4°C.

Techniques: Plasmid Preparation, Recombinant, Knock-Out, Blocking Assay, Staining, Antibody Labeling, Reverse Transcription, Lysis, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Fractionation, Cell Culture, Software, Flow Cytometry, Fluorescence, Spectrophotometry